Effect of the chicken zp1 gene on osteoblast mineralization / 生物工程学报

The aim of this study was to clone the chicken zp1 gene encoding zona pellucida 1 (Zp1) and investigate its tissues expression profile and its effect on osteoblast mineralization. The expression level of zp1 was quantified in various tissues of laying hens and in the tibia of the pre- and post-sexual maturity by RT-qPCR. Zp1 overexpressed vector was transfected into chicken calvarial osteoblasts which were induced differentiation for 8 days, and the extracellular mineral and the expression of mineralization-related genes were detected. The full-length chicken zp1 gene is 3 045 bp, encoding 958 amino acids residuals, and has two N-glycosylation sites. The highest expression level of the zp1 gene was found in the liver, followed by the tibia and yolk membrane, while no expression was detected in the heart and eggshell gland. Compared with the pre-sexual maturity hens, the concentration of estrogen (E2) in plasma, the content of glycosaminoglycan (GAG) and the expression level of the zp1 gene in the tibia with post-sexual maturity were higher. The extracellular matrix and the level of osteoblast mineralization-related genes showed a significantly upregulated expression in chicken calvarial osteoblasts with Zp1 overexpressed and addition of estrogen. The expression of the zp1 gene is tissue-specific and positively regulated osteoblast mineralization under the action of estrogen, laying the foundation for elucidating the functional properties of Zp1 in chicken bones during the egg production period.

Research Note: Effect of age on hematological parameter and reference intervals for commercial Lohmann silver layer.

Hematological parameters play a key role in assessing the disease states and physiological status of birds, however the reliable information of hematological reference values for Lohmann silver in the pre- and post-laying periods is limited. Thus, the aim of this study was to research the effect of age on hematological parameters and provide reliable reference intervals for commercial Lohmann silver layer. Blood samples were obtained from 231 laying hens at different ages (5-37 wk) with 4-wk sampling intervals. A total of 12 hematological parameters including leucocytes (WBC, 109/L), red blood cell (RBC,1012/L), hemoglobin (HGB, g/L), hematocrit (HCT,%), mean corpuscular volume (MCV, fL), mean corpuscular hemoglobin (MCH, pg), mean corpuscular hemoglobin concentration (MCHC, g/L), red cell distribution width (RDW,%), platelet count (PLT,109/L), mean platelet volume (MPV, fL), platelet volume distribution width (PDW), plateletcrit (PCT, fL) were determined with a reference value advisor. One-ANOVA and Tukey test were utilized to test for significant differences of each parameter between ages. Our findings showed that these parameters were significantly affected by age, except for HCT in the pre-laying period. And reference intervals for all parameters except MCH, MCHC, MCV, MPV, and PDW in the pre-laying period were higher than that in the post-laying period. The results of this study may serve as indispensable information when diagnosing the health of Lohmann silver layer.

Effect of housing system and age on products and bone properties of Taihang chickens.

The aim of this study was to compare egg quality, carcass, meat characteristics, and bone properties of Taihang chickens in 2 different housing systems at various ages. A total of 168 birds were selected and randomly allocated to 2 groups at 23 wks and raised in conventional cage (CC) or flattening on floor (FF) housing system, respectively. FF hens' egg weight, albumen height, and Haugh unit were higher (P < 0.05), and yolk weight was lower (P < 0.001) than those of the CC hens. Egg quantity of FF hens was higher than that of the CC hens (P < 0.01). The FF hens' weight (P < 0.05) and breast meat percent (P < 0.01) were higher than those of the CC hens. The highest live body and carcass weight were observed at 57 wk (both P < 0.01), whereas the highest semieviscerated percentage (P < 0.01) and meat weight of breast and thigh (P < 0.05) were shown at 49 wk. The highest eviscerated percentage and thigh meat were displayed at 41 and 32 wk, respectively (P < 0.01). For meat color, the lightness of both breast and thigh meat in the FF group was significantly reduced compared with those of the CC group (P < 0.01 and P < 0.05). FF hens' humerus weight and breaking strength (both P < 0.01) and tibia breaking strength (P < 0.05) were significantly higher than those of the CC hens. Femur breaking strength was significantly affected by hens' age (P < 0.01). Egg weight, albumen height, Haugh unit, yolk color (all P < 0.01), pH of thigh meat, semieviscerated, and eviscerated weight (all P < 0.05) were influenced by the interaction of housing system and age, whereas no change in moisture loss rate, meat color, shearing force, and bone quality was found (P > 0.05). In summary, in the 2 housing systems, hens' age and their interaction could affect slaughter performance, quality of egg, meat, and bone of Taihang chickens. In addition, the results of the present study support a theoretical basis for the development and utilization of Taihang chickens in accordance with the FF system.